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Objective: To detect the expression of P53, human epidermal growth factor receptor-2 (HER-2), and tumor endothelial marker 1 (TEM1) in gastric cancer tissues, analyze their correlation with clinical efficacy, and explore their potential roles as biomarkers for neoadjuvant chemotherapy. Methods: Sixty-three patients with gastric cancer who underwent fluorouracil-based neoadjuvant che-motherapy in The First Hospital of Lanzhou University from May 2015 to May 2017 were enrolled. Using immunohistochemistry, the expression of P53, Her2, and TEM1 was detected in 63 gastric cancer specimens before neoadjuvant chemotherapy. The efficacy of neoadjuvant chemotherapy was assessed by imaging. The relationship between the expression of P53, HER-2, and TEM1 and the effi-cacy of neoadjuvant chemotherapy was analyzed. Results: The total effective rate of neoadjuvant chemotherapy in 63 patients with advanced gastric cancer was 69.8%, with 2, 7, and 35 patients achieving complete remission, partial remission, and stable disease, re-spectively. Disease progression was noted in 19 patients. Univariate analysis revealed that patients positive for TEM1 and having high T stage had a poor response to neoadjuvant chemotherapy (P<0.05); furthermore, location, differentiation, and size of tumor; P53 posi-tivity (P=0.488); and Her-2 positivity (P=0.106) were not associated with the efficacy of neoadjuvant chemotherapy for gastric cancer. Multivariate analysis revealed that TEM1 positivity and a higher T stage could be factors that predicted the response to neoadjuvant chemotherapy in patients with advanced gastric cancer. Conclusions: TEM1, as a marker of tumor stroma, may be an important molec-ular biological indicator that predicts the poor response to neoadjuvant chemotherapy in patients with gastric cancer.
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Taurine-upregulated gene 1 (TUG1) is a recently identified oncogenic long non-coding RNA (lncRNA),that is overexpressed in various digestive system tumor tissues and cell lines,including esophageal cancer,gastric cancer,liver cancer,cholangiocarcinoma and biliary tract cancer,pancreatic cancer and colorectal cancer.Studies have shown that TUG1 participates in tumor cells proliferation,apoptosis,migration and invasion,and high expression of TUG1 is associated with clinicopathological features and prognosis of cancer patients,suggesting that lncRNA TUG1 is likely represents a feasible biomarker or therapeutic target in human digestive system cancers.
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more likely to have H.pylori infection than those without digestive symptoms.Conclusions The total infection rate of H.pylori in Chinese children and adolescents is 29% and there is significant difference in geographical distribution. The infection rate of H.pylori in the higher incidence area of gastric cancer is 2.8 times higher than that in the low incidence area of gastric cancer. However,high quality epidemiological investigation with large sample size is needed,in order to further clarify the details of H.pylori infection in children and adolescents in China.
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more likely to have H.pylori infection than those without digestive symptoms.Conclusions The total infection rate of H.pylori in Chinese children and adolescents is 29% and there is significant difference in geographical distribution. The infection rate of H.pylori in the higher incidence area of gastric cancer is 2.8 times higher than that in the low incidence area of gastric cancer. However,high quality epidemiological investigation with large sample size is needed,in order to further clarify the details of H.pylori infection in children and adolescents in China.
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Primary biliary cholangitis is an organ-specific chronic cholestatic autoimmune liver disease characterized by intrahepatic cholestasis, presence of anti-mitochondrial antibody in circulating blood, and progressive and non-suppurative damage of small intrahepatic bile ducts, which finally lead to extensive hepatic duct damage, biliary cirrhosis, and even liver failure. This article overviews the advances in the epidemiology, pathogenesis, diagnosis, and treatment of primary biliary cholangitis and points out that the safety and efficacy of the new drugs such as fibrates in the treatment of primary biliary cholangitis need to be confirmed by further studies.
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The intestinal microecosystem is composed of natural microflora, intestinal epithelial cells, and intestinal mucosal immune system. Nonalcoholic fatty liver disease (NAFLD) is a metabolic stress-induced liver injury associated with insulin resistance and genetic susceptibility. In recent years, there has been increasing evidence showing the involvement of imbalanced intestinal microflora in the pathogenesis of NAFLD. Overgrowth of intestinal microflora, increased permeability of intestinal mucosa, intestinal endotoxemia, and production of inflammatory cytokines play important roles in the development of NAFLD. Further studies on the relationship between intestinal microflora imbalance and the pathogenesis of NAFLD may shed light on the treatment and prevention of NAFLD.
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The intestinal microecosystem is composed of natural microflora,intestinal epithelial cells,and intestinal mucosal immune sys-tem.Nonalcoholic fatty liver disease (NAFLD)is a metabolic stress-induced liver injury associated with insulin resistance and genetic sus-ceptibility.In recent years,there has been increasing evidence showing the involvement of imbalanced intestinal microflora in the pathogene-sis of NAFLD.Overgrowth of intestinal microflora,increased permeability of intestinal mucosa,intestinal endotoxemia,and production of in-flammatory cytokines play important roles in the development of NAFLD.Further studies on the relationship between intestinal microflora im-balance and the pathogenesis of NAFLD may shed light on the treatment and prevention of NAFLD.
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The occurrence and outcome of cancer is related to the involved gene;Detecting the expres-sion of gene in body by the real-time PCR technology can diagnose and evaluate the prognosis of patients early. Through analyzing the abnormal genes and molecular,designing and studying the targeted drugs can achieve the therapeutic effect of steady,accurate,relentless,at the same time to reduce side effects and to improve the quality of life.
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Liver cancer is one of the most common malignant tumors in the world.The mortality of patients with liver cancer remains high e-ven with increasing progress in therapies.Early diagnosis is helpful for prolonging the survival of patients,so new biomarkers need to be ex-plored for timely diagnosis and treatment of liver cancer.The present research focuses on tumor-associated antigens (TAAs)and their cor-responding autoantibodies as serological biomarkers of liver cancer.The production of autoantibodies and the TAAs and anti-TAAs currently found in the liver cancer tissue are summarized.In addition,it is pointed out that these newly found autoantibodies have relatively high sen-sitivity and specificity for the early diagnosis of liver cancer.
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Background:To date,clinical studies on intravenous rabeprazole sodium for treatment of duodenobulbar ulcer bleeding are still lacking.Aims:To evaluate the efficacy and safety of intravenous rabeprazole sodium with different doses and times of administration in treating patients with duodenobulbar ulcer bleeding.Methods:A multicenter,randomized, double-blind,positive drug parallel-group controlled trial was performed.One hundred and five patients with duodenobulbar ulcer bleeding proved by gastroscopy were randomly divided into four groups.Patients in group A,B and C were treated with intravenous rabeprazole sodium 20 mg qd,40 mg qd and 20 mg bid for 5 days,respectively.Patients in control group received intravenous omeprazole sodium 40 mg bid for 5 days.Hemostatic rate was the primary endpoint,hemostatic time and amount of blood transfusion were the secondary endpoints.Results:Hemostatic rates in group A,B,C and control group were 96.2% (25 /26),92.6% (25 /27),100.0% (26 /26)and 100.0% (26 /26),respectively,no significant difference was seen between the four groups (P >0.05).Median hemostatic time in group A,B,C and control group were 24 (24,72)h,24 (24,72)h,24 (24,48)h and 24 (24,48)h,respectively,no significant difference was seen between the four groups (P >0.05).No patient need blood transfusion during the treatment course.Slight leucopenia was the exclusive adverse effect seen in one case in group C after accomplishment of treatment.Conclusions:Three intravenous rabeprazole sodium regimens with different doses and times of administration were all effective and safe for treatment of mild to moderate duodenobulbar ulcer bleeding.Administration with 20 mg bid seems more effective among the three regimens.
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Objective:This study was conducted to investigate Epstein-Barr virus (EBV) infection in gastric cancer in Wuwei ar-ea of Gansu province (China) and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas (EBVaGC). Meth-ods:Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-en-coded small RNAs (EBERs), miR-101, EZH2, and COX-2, in gastric cancer tissues (n=120) and the corresponding adjacent tissues (n=120). Results:The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric can-cer (EBVnGC) tissues were significant (P<0.05). The infection of EBV in gastric cancer tissues was positively related to miR-101 ex-pression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues (P<0.05). Conclusion:EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.
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Bevacizumab is an efficient and low-toxicity monoclonal antibody towards serum ventricular endothelial growth factor.At present,bevacizumab has been approved by FDA to use clinically for kinds of cancer,such as colorectal cancer,lung cancer,kidney cancer and breast cancer.As more and more patients received bevacizumab cliniclly,it's toxicities are also focused on increasingly.
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Objective:To investigate the expression of c-Jun and MMP-9 in gastric cancer tissues,para-cancerous tissues and metastastic lymph nodes,and to explore its role and significance for the clinicopathology and prognosis.Methods:Immunohistochemistry was employed to detect the expression of c-Jun and MMP-9 in tissue microarrays containing gastric normal mucosa(n=32),para-cancerous tissues(n=54),metastastic lymph nodes(n=41),and gastric cancer tissues(n=189).Results:The positive rates for c-Jun and MMP-9 expression in gastric cancer were 73.0%and 78.3%,respectively.The positive rates of c-Jun protein was significantly associated with the degree of differentiation(P<0.05),but was not associated with the depth of invasion,lymph node metastasis,Lauren type,sex,age or size of tumor(P>0.05).The positive rates of MMP-9 was significantly associated with the depth of invasion,lymph node metastasis,Lauren type and degree of differentiation(P<0.05),but was not associated with sex,age or size of tumor(P>0.05).The positive rates of MMP-9 expression in the 41 gastnc cancer tissue samples and 41 metastastic lymph node tissue samples were significantly different(P<0.05).In metastastic lymph nodes,the positive rate of MMP-9 expression was higher.Kaplan-Meier survival analysis showed that the survival rate of patients with negative c-Jun and MMP-9 expression was higher than that of patients with positive c-Jun and MMP-9 expression(P<0.05).COX regression analysis showed that c-Jun and MMP-9 expressioh was not independent prognostic factor for gastric cancer. Conclusion:The expression of c-Jun is positively associated with the degree of differentiation.The increased c-Jun expression maybe an early indicator of gastric Cancer. The high expression of MMP-9 may involve the Occurrence,development,invasion,and metastasis of gastric cancer. C-Jun and MMP-9 are useful markers for predicting the outcome of gastric cancer,but they are not independent prognostic factors.
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Objective E cadherin catenin complex mediates cell cell adhesion and plays an important role in maintaining normal architecture and function of epithelial tissues. We evaluated the expression patterns of E cadherin, ? catenin, and ? catenin in gastric carcinoma and determine the potential relationship between E cadherin catenin complex and tumor clinicopathological features as well as patient survival. Methods Immunohistochemical staining of E cadherin, ? catenin, and ? catenin in 148 gastric carcinomas was performed. Results The abnormal expression rates of E cadherin, ? catenin and ? catenin in the tissues of gastric carcinomas were 44.6%, 73.6% and 43.2%, respectively. The total abnormal expression rate of E cadherin complex in 148 cases of gastric carcinoma was 91.9%. Reduced expression of E cadherin, ? catenin and ? catenin were significantly correlated with tumor differentiation status ( P